Alternative names of antibody target: PMAIP1 , NOXA , APR
Background information: Noxa is a BH3-only member of the Bcl-2 family. Expression of Noxa gene involves direct activation of its promotor by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Coimmunoprecipitation data sμggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as Bcl-xL and Mcl-1. Blocking the endogenous Noxa induction results in the suppression of apoptosis. These sμggest Noxa may represent a candidate mediator of p53-induced apoptosis.
Antibody host: Rabbit
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
This antibody reacts with: Human, mouse, rat
Immunogene sequence: Synthetic peptide surrounding amino acid 100 of human Noxa
Concentration of antibody: 0.2 mg/ml
Form of antibody: Liquid
Formulation of this antibody: 100 µg (0.2mg/ml) protein A purified rabbit anti-Noxa polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.
Purification method: Affinity purified
This antibody has been succesfully tested for use in following methods: Western blot
Recommended usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes mainly ~16 kDa band in human, mouse and rat samples. A ~34 kDa band is also detected to the lesser extent. Reactivity to other species has not been tested.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
How to handle this antibody: The antibody solution should be gently mixed before use.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.