Long name
NCF1/p47 phox Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as
Anti-NCF1/p47 phox PAb ALEXA FLUOR 488
Category
Conjugated Primary Antibodies
Conjugated with
ALEXA FLUOR® 488
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NCF1/p47 phox
Specificity
This is a highly specific antibody against NCF1/p47 phox.
Modification
Unmodified
Modification Site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen range
165-210/390
Subcellular location
Cytoplasm
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NCF1
Gene ID Number
653361
Swiss Prot
P14598
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Mouse, Rat
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Purification
Purified by Protein A.
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-NCF1/p47 phox has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.