Long name
NCF1/p47 phox(Ser359) Antibody, ALEXA FLUOR 488 Conjugated
Also known as
Anti-NCF1/p47 phox Ser359 PAb ALEXA FLUOR 488
Category
Conjugated Primary Antibodies
Conjugated with
ALEXA FLUOR® 488
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NCF1/p47 phox Ser359
Specificity
This is a highly specific antibody against NCF1/p47 phox Ser359.
Modification
Phosphorylation
Modification Site
Ser359
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Source
KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359
Gene ID Number
653361
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Rat
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen
The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
Purification
Purified by Protein A.
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-NCF1/p47 phox Ser359 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.