Purity
NOX1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Ab raised in
Goat
Type
Polyclonal
Spicific to
This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
Antigen
NOX1 antibody was made against a 13 amino acid synthetic peptide near the internal region of NOX1.
Immuno application
ELISA, Western Blot
Antibody advice
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 65 kDa band observed in human and mouse colon lysates (calculated MW of 64.9 kDa according to NP_008983.2). The 65 kDa was also seen in cultured transgenic mouse Aortic Smooth muscle cells (MASM), but not to be seen at endogenous levels in MASM. Recommended concentration: 1-3 ug/mL.
Suspention, pH, azide
NOX1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Keep at
Aliquot and store at -20 grades C. Minimize freezing and thawing.
Works with
Human, Mouse
Omim nr
148536873
Ncbi nr
NP_008983.2, NP_39249.1
Vial with antibody
in solution
Quantity per ml
500 ug/mL
NCBI GENE nr
27035
GENE symbol
NOX1
Antigene name
NADPH oxidase 1
Source
Homo sapiens
Protein nr
Q9Y5S8
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.