Gene info
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox.Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. The NOH-1S currents were reversibly blocked by zinc, a known H+ channel inhibitor. The NOH-1S variant does not contain an electron transport chain and it is thought that H+ conductance is its main physiologic function, whereas NOH-1L may conduct H+ ions as part of its electron transport mechanism.
Gene names
NOX1, GP91-2, MOX1, NOH-1, NOH1
Antibodie's label
unlabelled serum
Purity
Antibody is purified by peptide affinity chromatography method.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOX1.
Immuno application
ELISA, Western Blot
Antibody advice
NOX1 antibody can be used for detection of NOX1 by ELISA at 1:62500. NOX1 antibody can be used for detection of NOX1 by western blot at 0.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store NOX1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
Works with
Human, Dog
Omim nr
148536873
Ncbi nr
NP_008983
KDa
65 kDa, 59 kDa
Vial with antibody
Lyophilized
Quantity per ml
1 mg/ml
NCBI GENE nr
27035
GENE symbol
NOX1
Antigene name
NADPH oxidase 1
Source
Homo sapiens
Protein nr
Q9Y5S8
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.