Gene info
CYBB is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD).Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Purity
Antibody is purified by peptide affinity chromatography method.
Ab raised in
Rabbit
Type
Polyclonal
Antigen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYBB.
Immuno application
ELISA, Western Blot
Antibody advice
CYBB antibody can be used for detection of CYBB by ELISA at 1:1562500. CYBB antibody can be used for detection of CYBB by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Keep at
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store CYBB antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
Works with
Human, Mouse, Rat
Omim nr
6996021
Ncbi nr
NP_000388
KDa
63 kDa
Vial with antibody
Lyophilized
Quantity per ml
1 mg/ml
NCBI GENE nr
1536
GENE symbol
CYBB
Antigene name
cytochrome b-245, beta polypeptide
Source
Homo sapiens
Protein nr
P04839
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.