Gene info The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
Purity NOX4 antibody is affinity chromatography purified via peptide column.
Ab raised in Rabbit
Spicific to NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Antigen NOX4 antibody was made against a 14 amino acid peptide near the amino terminus of human NOX4.
Immuno application ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
Antibody advice NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Suspention, pH, azide NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Keep at NOX4 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
Works with Human, Mouse, Rat
Omim nr 8393843
Ncbi nr NP_058627
KDa Predicted: 64 kDa Observed: 68 kDa
Vial with antibody in solution
Quantity per ml 1 mg/mL
NCBI GENE nr 50507
GENE symbol NOX4
Antigene name NADPH oxidase 4
Source homo sapiens
Properties If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.