Gene info
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
Purity
NOX4 antibody is affinity chromatography purified via peptide column.
Ab raised in
Rabbit
Type
Polyclonal
Immunoglobulin
IgG
Spicific to
NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Antigen
NOX4 antibody was made against a 14 amino acid peptide near the amino terminus of human NOX4.
Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
Antibody advice
NOX4 antibody can be used for detection of NOX4 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Suspention, pH, azide
NOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Keep at
NOX4 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
Works with
Human, Mouse, Rat
Omim nr
8393843
Ncbi nr
NP_058627
KDa
Predicted: 64 kDa Observed: 68 kDa
Vial with antibody
in solution
Quantity per ml
1 mg/mL
NCBI GENE nr
50507
GENE symbol
NOX4
Antigene name
NADPH oxidase 4
Source
homo sapiens
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.