Gene info
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX2, also known as cytochrome b beta (CYBB) is one of two proteins that make up Cytochrome b-245, thought to be a primary component of the microbicidal oxidase system of phagocytes. NOX2 deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD) (2). Activation of the NOX2 enzyme complex in microglia is thought to be neurotoxic and may play a role in Alzheimer’s and Parkinson’s disease (3).
Purity
NOX2 antibody is affinity chromatography purified via peptide column.
Ab raised in
Rabbit
Type
Polyclonal
Immunoglobulin
IgG
Spicific to
NOX2 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. NOX2 is predicted to not cross-react with other NOX proteins.
Antigen
Rabbit polyclonal NOX2 antibody was made against a 15 amino acid peptide near the amino terminus of human NOX2.
Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
Antibody advice
NOX2 antibody can be used for detection of NOX2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Suspention, pH, azide
NOX2 antibody is supplied in PBS containing 0.02% sodium azide.
Keep at
NOX2 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
Works with
Human, Mouse, Rat
Omim nr
6996021
Ncbi nr
NP_000388
KDa
Predicted: 59, 63 kDa Observed: 58 kDa
Vial with antibody
in solution
Quantity per ml
1 mg/mL
NCBI GENE nr
1536
GENE symbol
CYBB
Antigene name
cytochrome b-245, beta polypeptide
Source
homo sapiens
Protein nr
P04839
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.