Type of Immunogen NSF antibody (Biotin) was raised in Rabbit using recombinant rat Vesicle-fusing ATPase protein .
Raised in Rabbit
Cross Reactivity Rat
Method of Purification NSF antibody (Biotin) was purified by Ammonium Sulfate precipitation
Form & Buffer Provided with 0.03% Proclin 300, 50% Glycerol and 0.01M PBS, PH 7.4
Storage Store at 4 deg C for short term storage and -20 deg C for long term storage. Avoid repeat freeze/thaw cycles
Tested for ELISA; WB
Properties If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.