Type of Immunogen NPM antibodies were raised in mouse using recombinant human NPM (81-294aa) purified from E. coli ..
Raised in Mouse
Specificity Human
Cross Reactivity Should be determined by the end user.
Isotypes IgG2b kappa
Clone 5E3
Method of Purification NPM antibody was purified by protein-G affinity chromatography
Source Anti-human NPM antibody, clone 5E3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NPM protein.
Concentration 1 mg/ml
Form & Buffer Provided as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions Blue Ice
Tested for ELISA; IHC; WB
Usage Recommendations WB: 1:1,000-1:2,000
Properties If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.