Method of Purification Lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source Normal Human Serum/Plasma was obtained from healthy donors of US origin.
Concentration 60.0 mg/ml (lot specific)
Form & Buffer Lyophilized powder containing 10 mM Sodium Phosphate and 0.15 M Sodium Chloride. Protease/IgG free, pH 7.2 Rehydrate with 2.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Storage Store sealed lyophilized serum at 4 deg C. Once open, store lyophilized at -20 deg C, reconstituted at 2-8 deg C (preservative added), or aliquoted at -20 deg C (no preservative added).
Shipping conditions Blue Ice
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.