Method of Purification Lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Source Normal Horse Serum was obtained from healthy donors of US origin.
Concentration 60.0 mg/ml (lot specific)
Form & Buffer Lyophilized powder containing 10 mM Sodium Phosphate and 0.15 M Sodium Chloride. Protease/IgG free, pH 7.2 Rehydrate with 2.0 ml of deionized water. Swirl gentle and let stand for up to 2 hours at 18-25 °C. Centrifuge reconstituted serum to remove any precipitates.
Storage Store sealed lyophilized serum at 4 deg C. Once open, store lyophilized at -20 deg C, reconstituted at 2-8 deg C (preservative added), or aliquoted at -20 deg C (no preservative added).
Shipping conditions Blue Ice
Properties Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.