Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) CLIA Kit, 96 well plate

  • Catalog name: EKU09954
  • Supplier name: bioma
  • Size: 96T
  • Price: 1092.00€
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  • Verified reactivity Rattus norvegicus (Rat)
  • Detection limits 312.50-20000 pg/mL
  • Sensitivity limit 11,88 pg/mL
  • Samples to be analyzed Tissue homogenates and other biological fluids
  • Assay duration 2 hours, 40min
  • Verified applications CLIA
  • Assay class Sandwich
  • ELISA detection Chemiluminescent
  • Protein number Q9WV87
  • Alternate protein number Please refer to SwissProt
  • Gene number 114243
  • Gene name Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1
  • Alternate gene name MOX1; GP91-2; NOH1; NADPH Oxidase 1; Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating; NADH/NADPH mitogenic oxidase subunit P65-MOX
  • Estimated production time 7-11 business days
  • Shipping requirements Blue ice
  • Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for 8 months
  • ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
  • ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle The microplate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) level in the sample or standard.;
  • Protocol Please see ELISA's datasheet, otherwise contact us
  • Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area Enzyme & Kinase;Neuro science;Developmental science;
  • Warnings Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes For research use only. Not for diagnostic procedures.
  • About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name Rattus norvegicus