Mouse Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) ELISA Kit, 96 well plate

  • Catalog name: EKU06294
  • Supplier name: bioma
  • Size: 10x96T
  • Price: 6376.00€
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  • Verified reactivity Mus musculus (Mouse)
  • Detection limits 0.156-10 ng/mL
  • Sensitivity limit 0,058 ng/mL
  • Samples to be analyzed Tissue homogenates, cell lysates and other biological fluids
  • Assay duration 3 hours
  • Verified applications ELISA
  • Assay class Sandwich
  • ELISA detection Colorimetric
  • Protein number Please refer to Uniprot
  • Alternate protein number Please refer to SwissProt
  • Gene number Please refer to GenBank
  • Gene name Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4
  • Alternate gene name KOX; KOX-1; RENOX; NADPH Oxidase 4
  • Estimated production time 7-11 business days
  • Shipping requirements Blue ice
  • Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for 8 months
  • ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) and analogues was observed.
  • ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Protocol Please see ELISA's datasheet, otherwise contact us
  • Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 (NOX4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area Enzyme & Kinase;
  • Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes For research use only. Not for diagnostic procedures.
  • Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name Mus musculus