Rat Dual Oxidase 2 (DUOX2) ELISA Kit, 96 well plate

  • Catalog name: EKU03796
  • Supplier name: bioma
  • Size: 5x96T
  • Price: 3868.00€
  • Check this product
  • Verified reactivity Rattus norvegicus (Rat)
  • Detection limits 0.156-10 ng/mL
  • Sensitivity limit 0,063 ng/mL,
  • Samples to be analyzed Serum, plasma, tissue homogenates and other biological fluids.
  • Assay duration 3 hours
  • Verified applications ELISA
  • Assay class Sandwich
  • ELISA detection Colorimetric
  • Protein number Q9ES45
  • Alternate protein number Please refer to SwissProt
  • Gene number 79107
  • Gene name Dual Oxidase 2
  • Alternate gene name LNOX2; NOXEF2; THOX2; Thyroid oxidase 2; NADH/NADPH thyroid oxidase p138-tox; Dual Oxidase-Like Domains 2; Nicotinamide Adenine Dinucleotide Phosphate Oxidase
  • Estimated production time 7-11 business days
  • Shipping requirements Blue ice
  • Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for 8 months
  • ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Dual Oxidase 2 (DUOX2). No significant cross-reactivity or interference between Dual Oxidase 2 (DUOX2) and analogues was observed.
  • ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Oxidase 2 (DUOX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Oxidase 2 (DUOX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Oxidase 2 (DUOX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Oxidase 2 (DUOX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Protocol Please see ELISA's datasheet, otherwise contact us
  • Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dual Oxidase 2 (DUOX2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area Enzyme & Kinase;
  • Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes For research use only. Not for diagnostic procedures.
  • Test The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name Rattus norvegicus