Application
For research use only. Not for diagnostic procedures.
Assay Type
Competitive
Shipping Conditions
Ice packs
Storage Temperature
Short term: 4°C; Long term: see manual.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Rat NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit before 6 months
Specificity
NA
Cross Activity
No significant cross-reactivity or interference was observed.
Test Principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat NADPH. During the reaction, Rat NADPH in the sample or standard competes with a fixed amount of Rat NADPH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat NADPH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat NADPH in the samples is then determined by comparing the OD of the samples to the standard curve.
Stability
NA
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
NA
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.