Long name
Recombinant Anoxybacillus flavithermus Biotin synthase (bioB)
Alternative names
biotin synthase; Biotin synthase; biotin synthase;
Gene name
bioB
Other gene names
bioB; bioB;
General description
Biotin synthase (bioB) is a recombinant protein expressed in Mammalian Cell . The protein can be with or without a His-Tag or other tag in accordance to customer's request. All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility.
Product category
Recombinant Proteins
Expression system
Mammalian Cell
Available also expressed in:
E Coli ; Yeast ; Baculovirus ; Mammalian Cell
Purity
Greater than 90% (determined by SDS-PAGE)
Form
Lyophilized protein
Storage
This protein can be stored at -20 degrees Celsius. For extended periods of time it is recommended to keep the protein frozen at -40 or -80 degrees Celsius. Avoid cycles of freezing and thawing as they might denaturate the polypeptide chains.
Applications
This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.