Specificity This antibody reacts specifically with Cyclooxygenase 3
Modification No modification has been applied to this antibody
Modification site None
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Subcellular locations N/A
Antigen Source KLH conjugated synthetic peptide derived from human Cyclooxygenase 3/Cox3
Gene ID N/A
Swiss Prot N/A
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information Cytochrome c oxidase subunit III, COX3 (also designated COIII, MTCO3 or oxidative phosphorylation (OxPhos) Complex IV, subunit III) is one of three mitochondrial DNA (mtDNA) encoded subunits (MTCO1-3) of respiratory Complex IV. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electro-chemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. Apoptosis in mammalian cells may be triggered by inhibition of mitochondrial COX. Specifically, reduction of COX3 has been associated with apoptosis and studies have shown that overexpression of COX3 results in a reduction of signals associated with apoptosis.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Also known as Cyclooxygenase 3 Polyclonal Antibody
Other name Anti-Cyclooxygenase 3 Polyclonal
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.