Specificity This antibody reacts specifically with DNCLI1 ALEXA FLUOR® 647
Modification No modification has been applied to this antibody
Modification site None
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Subcellular locations Cytoplasm
Antigen Source KLH conjugated synthetic peptide derived from human DNCLI1
Gene ID 51143
Swiss Prot Q9Y6G9
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Background information Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Also known as DNCLI1 Polyclonal Antibody ALEXA FLUOR® 647
Other name Anti-DNCLI1 Polyclonal ALEXA FLUOR® 647
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Properties For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Polyclonal Antibodies made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-DNCLI1 (Polyclonal) exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.
Conjugation Alexa Fluor,ALEXA FLUOR 647
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.