Anti-P53(Ser392) (Polyclonal), ALEXA Fluor 594

  • Catalog name: GENTObs-1650R-A594
  • Supplier name: Bioss Polyclonal Antibodies
  • Size: 100 microliters
  • Price: 516.00€
  • Type Conjugated Primary Antibody
  • Conjugated with ALEXA FLUOR® 594
  • Host organism Rabbit (Oryctolagus cuniculus)
  • Target Protein/Peptide P53 Ser392
  • Specificity This antibody reacts specifically with P53(Ser392)
  • Modification Phosphorylation
  • Modification site Ser392
  • Clonality Polyclonal Antibody
  • Clone Polyclonal Antibodies
  • Concentration 1ug per 1ul
  • Subcellular locations N/A
  • Antigen Source KLH conjugated synthetic phosphopeptide derived from human p53 around the phosphorylation site of (Ser392)
  • Gene ID 7157
  • Swiss Prot P04637
  • Applications IF(IHC-P)
  • Applications with corresponding dilutions IF(IHC-P)(1:50-200)
  • Cross reactive species Human (Homo sapiens)
  • Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
  • Background information Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
  • Purification method Purified by Protein A.
  • Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
  • Excitation emission 590nm/617nm
  • Synonyms P53; BCC7; LFS1; TRP53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53
  • Also known as P53(Ser392) Polyclonal Antibody
  • Other name Anti-P53(Ser392) Polyclonal
  • Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
  • Properties For facs or microscopy Alexa 1 conjugate.
  • Conjugation Alexa Fluor,ALEXA FLUOR® 594
  • Conjugated Alexa conjugate 1
  • Group Polyclonals and antibodies
  • About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.