Long name P105 RB(Ser612) Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
Also known as Anti-P105 RB Ser612 PAb ALEXA FLUOR 350
Category Conjugated Primary Antibodies
Conjugated with ALEXA FLUOR® 350
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen P105 RB Ser612
Specificity This is a highly specific antibody against P105 RB Ser612.
Modification Site Ser612
Clone Polyclonal antibody
Concentration 1ug per 1ul
Source KLH conjugated synthetic phosphopeptide derived from human Retinoblastoma associated protein around the phosphorylation site of Ser612
Gene ID Number 5925
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactivity Human, Mouse, Rat
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties For facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.