Long name phospho Met/C-Met(Tyr1365) Antibody, ALEXA FLUOR 594 Conjugated
Also known as Anti-c-Met Tyr1365 PAb ALEXA FLUOR 594
Category Conjugated Primary Antibodies
Conjugated with ALEXA FLUOR® 594
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen c-Met Tyr1365
Specificity This is a highly specific antibody against c-Met Tyr1365.
Modification Site Tyr1365
Clone Polyclonal antibody
Concentration 1ug per 1ul
Immunogen range 1345-1385/1390
Subcellular location Cytoplasm
Source KLH conjugated synthetic phosphopeptide derived from human C-Met around the phosphorylation site of Tyr1365
Gene ID Number 4233
Swiss Prot P08581
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactivity Human, Mouse, Rat
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties For facs or microscopy Alexa 1 conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.