c-Raf(Tyr341) Antibody, ALEXA FLUOR 350

  • Catalog name: bs-5650R-A350
  • Supplier name: Bioss Primary Conjugated Antibodies. ALEXA FLUOR
  • Size: 100ul
  • Price: 350.15€
  • Long name c-Raf(Tyr341) Polyclonal Antibody, ALEXA FLUOR 350 Conjugated
  • Also known as Anti-c-Raf Tyr341 PAb ALEXA FLUOR 350
  • Category Conjugated Primary Antibodies
  • Conjugated with ALEXA FLUOR® 350
  • Host Organism Rabbit (Oryctolagus cuniculus)
  • Target Antigen c-Raf Tyr341
  • Specificity This is a highly specific antibody against c-Raf Tyr341.
  • Modification Phosphorylation
  • Modification Site Tyr341
  • Clonality Polyclonal
  • Clone Polyclonal antibody
  • Concentration 1ug per 1ul
  • Immunogen range 325-375/648
  • Subcellular location Cytoplasm, Nucleus, Mitochondrion, Cell membrane
  • Source KLH conjugated synthetic phosphopeptide derived from human c-Raf around the phosphorylation site of Tyr341
  • Gene ID Number 5894
  • Swiss Prot P04049
  • Tested applications IF(IHC-P)
  • Recommended dilutions IF(IHC-P)(1:50-200)
  • Crossreactivity Human
  • Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Background of the antigen Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
  • Purification Purified by Protein A.
  • Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
  • Excitation emission 343nm/442nm
  • Synonyms NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; RAF1; RAF
  • Properties For facs or microscopy Alexa 1 conjugate.Alexa Fluor 350 conjugates can be used in multi-color flow cytometry with FACS's equipped with a second red laser or red diode.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Conjugation Alexa Fluor,ALEXA FLUOR 350
  • French translation anticorps