Long name Dab1 (Ser524) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as Anti-Dab1 Ser524 PAb ALEXA FLUOR 488
Category Conjugated Primary Antibodies
Conjugated with ALEXA FLUOR® 488
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen Dab1 Ser524
Specificity This is a highly specific antibody against Dab1 Ser524.
Modification Site Ser524
Clone Polyclonal antibody
Concentration 1ug per 1ul
Source KLH conjugated synthetic phosphopeptide derived from human Dab1 around the phosphorylation site of Ser524
Gene ID Number 1600
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactivity Human, Mouse, Rat
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen Disabled 1 (Dab1) is an 80 kDa protein that is encoded by the Disabled-1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases. Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 serine 491 is phosphorylated in a Cdk5-dependent manner and regulates, likely indirectly, Reelin-induced signaling during neural cortex development.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Properties For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Dab1 (Ser524) Antibody, has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.