Long name SEMA3E/Semaphorin 3E Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
Also known as Anti-SEMA3E/Semaphorin 3E PAb ALEXA FLUOR 555
Category Conjugated Primary Antibodies
Conjugated with ALEXA FLUOR® 555
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen SEMA3E/Semaphorin 3E
Specificity This is a highly specific antibody against SEMA3E/Semaphorin 3E.
Modification Site None
Clone Polyclonal antibody
Concentration 1ug per 1ul
Source This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human SEMA3E/Semaphorin 3E
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactivity Human, Mouse, Rat
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen Semaphorins are a family of cell surface and secreted proteins involved in neural development that are conserved from insects to humans. Members of this family are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular â€œsemaphorinâ€ domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3E is a secreted semaphorin with 775 amino acids. Mutations in the SEMA3E gene are associated with CHARGE syndrome, a disorder characterized by cranial nerve dysfunction, coloboma of the eye, choanal atresia, inner and external ear abnormalities, cardiac anomalies, genitourinary abnormalities, and growth retardation.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission 553nm/568nm
Synonyms Coll 5; Coll5; KIAA0331; M sema H; M SEMAH; M SemaK; Msema H; MSEMAH; MSemaK; SEM3E_HUMAN; Sema domain immunoglobulin domain Ig; Sema domain immunoglobulin domain Ig short basic domain secreted 3E; Sema domain, immunoglobulin domain Ig, short basic domain, secreted, semaphorin 3E; SEMA3E; SEMAH; Semaphorin 3E; Semaphorin-3E; Semaphorin3E; Short basic domain secreted semaphorin 3E; SEM3E_HUMAN.
Properties For facs or microscopy Alexa 1 conjugate.Very high photo stable ALEXA conjugate.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.