Long name TRIM25(Thr91) Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Also known as Anti-TRIM25 Thr91 PAb ALEXA FLUOR 488
Category Conjugated Primary Antibodies
Conjugated with ALEXA FLUOR® 488
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen TRIM25 Thr91
Specificity This is a highly specific antibody against TRIM25 Thr91.
Modification Site Thr91
Clone Polyclonal antibody
Concentration 1ug per 1ul
Source KLH conjugated synthetic phosphopeptide derived from human TRIM25 around the phosphorylation site of Thr91
Gene ID Number 7706
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Cross-reactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Background of the antigen The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.
Purification Purified by Protein A.
Storage conditions Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Excitation emission 499nm/519nm
Synonyms TRIM25phospho T91ï¼›EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite mot containing 25; Tripartite mot containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 estrogen responsive finger protein; ZNF 147; ZNF147.
Properties For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the TRIM25(Thr91) Antibody, has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.