Water-Soluble Tetrazolium Cell Proliferation Assay Kit

  • Catalog name: X2833K
  • Supplier name: nordc
  • Size: 500 Tests
  • Price: 168.00€
  • Category Other Reagents
  • Long description WST-8 Cell Proliferation Kits allows very convenient assays by utilizing ExalphaÕs highly water-soluble tetrazolium salt. WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H- tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator, as shown in Figure 1._x000B__x000B_WST-8 is a one-bottle solution; no premixing of components is required. WST-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium (Figure 2). The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells._x000B__x000B_Figure 3 shows that the cell proliferation assay using WST-8 correlates well with the [3H]-thymidine incorporation assay. Thus, the WST-8 assay can also be substituted for the [3H]-thymidine incorporation assay. As shown in Figure 4, the detection sensitivity using WST-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
  • Antibody come from n/a
  • Other description No other information available
  • Clone not specified
  • Antigen-antibody binding interaction Water-Soluble Tetrazolium Cell Proliferation Assay Kit
  • Antibody is raised in see techfile
  • Antibody's reacts with see techfile
  • Antibody's reacts with these species This antibody doesn't cross react with other species
  • Antibody's specificity No Data Available
  • Application not tested yet / to be tested
  • Antibody's suited for contact us
  • Storage -20ºC
  • Relevant references no information yet
  • Protein number see ncbi
  • Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Tissue cell
  • Test nordc supplies other types of Assays as 1.