Long description Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are -tubulin and -tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of - and -tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8._x000B__x000B_Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be related to tubulin._x000B__x000B_To form microtubules, the dimers of - and -tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Whether the -tubulin member of the tubulin dimer is bound to GTP or GDP influences the stability of the dimer in the microtubule. -tubulin faces the plus end of the microtubule while -tubulin faces the minus end. That is, the (-) side of added dimers connect to the plus end whereas the sides (+) point outwards and connect to incoming minus ends._x000B__x000B_Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart; thus, this GTP cycle is essential for the dynamic instability of the microtubule.
Antibody come from Synthetic peptide derived from the human PINK1 protein. Control for antibody to G309D mutant (Cat. No. X2761P)
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
Antigen-antibody binding interaction Mouse anti beta-Tubilin Antibody
Antibody is raised in Mouse
Antibody's reacts with Human, Mouse, rat, monkey, rabbit, chicken
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Application WB, IS, ELISA, DOT BLOT
Antibody's suited for Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Matsuda, N., et al. ÔPINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy.Õ J. Cell. Biol., 189, 211-221 (2010)_x000B__x000B_2. Vives-Bauza, C., et al. ÔPINK1-dependent recruitment of Parkin to mitochondria in mitophagy.Õ Proc. Natl. Acad. Sci. USA, 107, 378-383 (2010)_x000B__x000B_3. Valente, E.M., et al. ÔPINK1 mutations are associated with sporadic early-onset parkinsonism.Õ Ann. Neurol., 56, 336-341 (2004)_x000B__x000B_4. Geisler, S., et al. ÔThe PINK1/Parkin-mediated mitophagy is compromised by PD-associated mutations.Õ Autophagy, 6, 871-878 (2010)_x000B__x000B_5. Valente, E.M., et al. ÔHereditary early-onset Parkinson's disease caused by mutations in PINK1.Õ Science, 304, 1158-1160 (2004)_x000B__x000B_6. Silvestri, L., et al. ÔMitochondrial import and enzymatic activity of PINK1 mutants associated to recessive parkinsonism.Õ Hum. Mol. Genet., 14, 3477-3492 (2005)
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.