Long description Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells.
Antibody come from Synthetic peptide derived from a mutant form of the human PINK1 protein
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Antibody's suited for Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Matsuda, N., et al. ÔPINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy.Õ J. Cell. Biol., 189, 211-221 (2010)_x000B__x000B_2. Vives-Bauza, C., et al. ÔPINK1-dependent recruitment of Parkin to mitochondria in mitophagy.Õ Proc. Natl. Acad. Sci. USA, 107, 378-383 (2010)_x000B__x000B_3. Valente, E.M., et al. ÔPINK1 mutations are associated with sporadic early-onset parkinsonism.Õ Ann. Neurol., 56, 336-341 (2004)_x000B__x000B_4. Geisler, S., et al. ÔThe PINK1/Parkin-mediated mitophagy is compromised by PD-associated mutations.Õ Autophagy, 6, 871-878 (2010)_x000B__x000B_5. Valente, E.M., et al. ÔHereditary early-onset Parkinson's disease caused by mutations in PINK1.Õ Science, 304, 1158-1160 (2004)_x000B__x000B_6. Silvestri, L., et al. ÔMitochondrial import and enzymatic activity of PINK1 mutants associated to recessive parkinsonism.Õ Hum. Mol. Genet., 14, 3477-3492 (2005)
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.