Long description Recognizes a protein of 33-36kDa, identified as the Excision Repair Cross Complementing (ERCC1). The mammalian ERCC1-encoded polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. The structure-specific ERCC1/XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV-induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross-links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
Antibody come from Synthetic peptide derived from human histone H2a.x protein.
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffinity Purification
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Application Western Blot, Immunohistochemistry (paraffin-embedded)
Antibody's suited for Antibody can be used for Western blotting (1-5 µg/ml) and immunohistochemistry (2-5 µg/ml at pH 9.5). Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Paull, T.T., et al. ÔA critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damageÕ Curr. Biol., 10, 886-895 (2000)_x000B__x000B_2. Kobayashi, J., et al. ÔNBS1 localizes to gamma-H2AX foci through interaction with the FHA/BRCT domain.Õ Curr. Biol., 12, 1846-1851 (2002)_x000B__x000B_3. Stewart, G.S., et al. ÔMDC1 is a mediator of the mammalian DNA damage checkpoint.Õ Nature, 421, 961-966 (2003)_x000B__x000B_4. Lukas, C., et al. ÔMdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention.Õ EMBO J., 23, 2674-2683 (2004)
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
About Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.