Long description Receptor for ADP. Coupled to G(i)-proteins. May play a role in hematopoiesis and the immune system. Strong expression in spleen and adult brain. Lower expression in placenta, lung, liver, spinal cord, thymus, small intestine, uterus, stomach, testis, fetal brain, and adrenal gland. Not detected in pancreas, heart, kidney, skeletal muscle, ovary or fetal aorta. Clearly detected in lymph node and bone marrow, weakly detected in peripheral blood mononuclear cells (PBMC) and in peripheral blood leukocytes (PBL), but not detected in polymorphonuclear cells (PMN). In the brain, detected in all brain regions examined. Stimulation by ADP in stably transfected CHO cells resulted in inhibition of adenylyl cyclase and the phosphorylation of the MAP kinases MAPK3 and MAPK1 in a pertussis toxin-sensitive way. Inhibition of adenylyl cyclase and phosphorylation of the MAP kinases are transduction mechanisms that involve G(i) proteins.
Antibody come from Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffiinity Purification
Antigen-antibody binding interaction Rabbit anti G-Protein Coupled Receptor 94 Antibody
Antibody is raised in Rabbit
Antibody's reacts with Human
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Application Western blot
Antibody's suited for This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 µg/mg of protein lysate), Western blotting (1 µg/ml) and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Thulasi R; et al. Alpha 2a-interferon-induced differentiation of human alveolar rhabdomyosarcoma cells: correlation with down-regulation of the insulin-like growth factor type I receptor. Cell Growth and Differentiation, 1996 Apr, 7(4):531-41._x000B__x000B_2. Wesche WA; et al. Immunohistochemistry of MyoD1 in adult pleomorphic soft tissue sarcomas. American Journal of Surgical Pathology, 1995, 19(3):261-9._x000B__x000B_3. Parham DM; et al. Immunohistochemical analysis of the distribution of MyoD1 in muscle biopsies of primary myopathies and neurogenic atrophy. Acta Neuropathologica, 1994, 87:605-11._x000B__x000B_4. Tallini G; et al. Myogenic regulatory protein expression in adult soft tissue sarcomas. A sensitive and specific marker of skeletal muscle differentiation. American Journal of Pathology, 1994 Apr, 144(4):693-701._x000B__x000B_5. Dias P; et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Research, 1992 Dec 1, 52(23):6431-9._x000B__x000B_6. Rosai J; et al. MyoD1 protein expression in alveolar soft part sarcoma as confirmatory evidence of its skeletal muscle nature. American Journal of Surgical Pathology, 1991 Oct, 15(10):974-81._x000B_
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
About Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.