Long description Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Antibody come from Synthetic peptide derived from human Numb protein
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
Antigen-antibody binding interaction Rabbit anti FBXW7 Antibody
Antibody is raised in Rabbit
Antibody's reacts with Human
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Application Western Blot
Antibody's suited for Antibody can be used for Western blotting (1:400 starting dilution) and immunohistochemistry (10-15 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Susini, L., et al. 'Siah-1 binds and regulates the function of Numb.; Proc. Natl. Acad. Sci. U.S.A. 98:15067-15072(2001)._x000B__x000B_2. Verdi, J.M., et al. 'Distinct human NUMB isoforms regulate differentiation vs. proliferation in the neuronal lineage.; Proc. Natl. Acad. Sci. U.S.A. 96:10472-10476(1999).
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
About Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.