Long description Sphingomyelin Synthase is a bidirectional lipid cholinephosphotransferases capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) in addition to performing the inverse reaction. The direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. It directly and specifically recognizes the choline head group on the substrate. It also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. It may not function strictly as a SM synthase and is inhibited by bacterial PC-phospholipase C inhibitor D609. It is localized to Golgi apparatus; Golgi membrane; multi-pass membrane protein. It can be possibly present on the brain, heart, kidney, liver, muscle and stomach.
Antibody come from Synthetic peptide derived from the human NHE-1 protein
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Application western blot_x000B_
Antibody's suited for Antibody can be used for Western blotting (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Relevant references 1. Pang, T., et al. 'Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger.; J. Biol. Chem. 277:43771-43777(2002)._x000B__x000B_2. Slepkov, E.R., et al. 'Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1.; Biochem. J. 379:31-38(2004)._x000B__x000B_3. Slepkov, E.R., et al. 'Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger.; J. Biol. Chem. 280:17863-17872(2005).
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Test You can block the antibody by the specific target amino acid sequence of peptide.