Alternative Name
NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX
Immunogen
A synthetic peptide corresponding to residues in Human NOX4.
Swiss-Prot
Q9NPH5
Reactivity
Human,Mouse,Rat
Applications
WB
Application_all
WB: 1:500~1:1000_x000D_
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity
NOX4 polyclonal antibody detects endogenous levels of NOX4 protein.
Usage
Western blot (WB) analysis of NOX4 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)
BiowMW
~ 67 kDa
Note
For research use only, not for use in diagnostic procedure.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.