Composition (g/L) Proteose peptone 40.000 Disodium phosphate 5.000 Monopotassium phosphate 1.000 Sodium chloride 2.000 Magnesium sulphate 0.100 Glucose 2.000 Hemin 0.005 Agar 25.000 Final pH ( at 25°C) 7.6±0.2
Quantity required per litre 75,10 g
Appearance Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium Basal medium: Medium amber coloured, clear to slightly opalescent gel After addition of egg yolk emulsion (FD045): Yellow coloured opaque gel forms in Petri plates
Cultural response Cultural characteristics observed with added Egg yolk emulsion (FD045),after an incubation at 35-37°C for 48-72 hours when incubated anaerobically. (Plates should be incubated up to 7 days before regarding them as negative)
Storage Store below 30°C in tightly closed container and use the prepared medium as fresh as possible.