Antigen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated.
Immuno application ELISA, Western Blot
Antibody advice ELISA:Direct:To detect h4-1BBL by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL.SandwichTo detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Keep at 4-1BBL antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Works with Human
Omim nr 728739
Ncbi nr P41273
Vial with antibody Lyophilized
NCBI GENE nr 8744
GENE symbol TNFSF9
Antigene name tumor necrosis factor (ligand) superfamily, member 9
Source Homo sapiens
Protein nr P41273
Properties If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.