NADPH oxidase 4 Antibody

  • Catalog name: PA1929
  • Supplier name: boster
  • Size: 0,1 mg
  • Price: 320.00€
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  • Target antigen NADPH oxidase 4
  • Clonality Polyclonal antibody
  • Clone Polyclonal antibody
  • Raised in rabbit
  • Type of the antibody IgG polyclonal antibody
  • Product form freeze-dried
  • Reacts with species: human Predicted to work with: mouse, rat
  • Analyses WB,IHC-P
  • Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.
  • Product configuration Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
  • Purification Immunogen affinity purified.
  • Solubilization The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
  • Storage condtions Keep the NADPH oxidase 4 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
  • Tips The NADPH oxidase 4 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
  • Background NOX4(NADPH oxidase 4), also called RENAL NADPH OXIDASE or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al.(2000) stated that the nucleotide sequence of RENOX matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to RENOX. By FISH, Shiose et al.(2001) mapped the NOX4 gene to chromosome 11q14.2-q21. Geiszt et al.(2000) found that NIH 3T3 fibroblasts overexpressing transfected RENOX showed increased production of superoxide and developed signs of cellular senescence. They suggested that RENOX, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al.(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.
  • Related articles 1. Ago, T., Kitazono, T., Ooboshi, H., Iyama, T., Han, Y. H., Takada, J., Wakisaka, M., Ibayashi, S., Utsumi, H., Iida, M. Nox4 as the major catalytic component of an endothelial NAD(P)H oxidase. Circulation 109: 227-233, 2004. 2. Geiszt, M., Kopp, J. B., Varnai, P., Leto, T. L. Identification of Renox, an NAD(P)H oxidase in kidney. Proc. Nat. Acad. Sci. 97: 8010-8014, 2000. 3. Shiose, A., Kuroda, J., Tsuruya, K., Hirai, M., Hirakata, H., Naito, S., Hattori, M., Sakaki Y., Sumimoto, H. A novel superoxide-producing NAD(P)H oxidase in kidney. J. Biol. Chem. 276: 1417-1423, 2001.
  • Gene Name NOX4
  • Protein Name NADPH oxidase 4
  • Gene Full Name NADPH oxidase 4
  • Synonyms Kidney oxidase-1 antibody|Kidney superoxide-producing NADPH oxidase antibody|KOX 1 antibody|KOX antibody|Kox-1 antibody|NADPH antibody|NADPH oxidase 4 antibody|Nox4 antibody|NOX4_HUMAN antibody|Renal NAD(P)H-oxidase antibody|RENOX antibody
  • Uniprot ID Q9JHI8
  • Properties If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation anticorps