Gene info
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).
Purity
NOX1 antibody is affinity chromatography purified via peptide column.
Ab raised in
Rabbit
Type
Polyclonal
Immunoglobulin
IgG
Spicific to
NOX1 antibody is human specific.
Antigen
NOX1 antibody was made against a 15 amino acid peptide near the center of human NOX1.
Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
Antibody advice
NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL.
Suspention, pH, azide
NOX1 antibody is supplied in PBS containing 0.02% sodium azide.
Keep at
NOX1 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.
Works with
Human
Omim nr
148536873
Ncbi nr
NP_008983
KDa
Predicted: 62 kDa Observed: 64 kDa
Vial with antibody
in solution
Quantity per ml
1 mg/mL
NCBI GENE nr
27035
GENE symbol
NOX1
Antigene name
NADPH oxidase 1
Source
homo sapiens
Protein nr
Q9Y5S8
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.