Human DUOX2(Dual Oxidase 2) ELISA Kit

  • Catalog name: ELK3846
  • Supplier name: ELK Biotech
  • Size: 96T
  • Price: 608.00€
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  • Alternative Names LNOX2; NOXEF2; THOX2; Thyroid oxidase 2; NADH/NADPH thyroid oxidase p138-tox; Dual Oxidase-Like Domains 2; Nicotinamide Adenine Dinucleotide Phosphate Oxidase
  • Assay Type Sandwich
  • Sensitivity 0.059ng/mL
  • Standard 10ng/mL
  • Detection range 0.156-10ng/mL
  • Assay length 3h
  • Research Area Enzyme & Kinase;Endocrinology;Hormone metabolism;
  • Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Oxidase 2 (DUOX2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Oxidase 2 (DUOX2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Oxidase 2 (DUOX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dual Oxidase 2 (DUOX2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Test The duality of this product lies in it's dual specificity to the gene, pH or protein. Mainly in phosphorylation by specific phosphatases.ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
  • Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.