Long name NOX5 Polyclonal Antibody, FITC Conjugated
Category Conjugated Primary Antibodies
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen NOX5
Specificity This is a highly specific antibody against NOX5.
Modification site None
Clone Polyclonal antibody
Concentration 1ug per 1ul
Subcellular location Mitochondrion
Source KLH conjugated synthetic peptide derived from human NOX5
Gene ID number 339345
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis.
Purification method This antibody was purified via Protein A.
Storage conditions Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Properties If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.