Long name
NCF1/p47 phox Polyclonal Antibody, FITC Conjugated
Category
Conjugated Primary Antibodies
Conjugation
FITC
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
NCF1/p47 phox
Specificity
This is a highly specific antibody against NCF1/p47 phox.
Modification
Unmodified
Modification site
None
Clonality
Polyclonal
Clone
Polyclonal antibody
Concentration
1ug per 1ul
Immunogen_range
165-210/390
Subcellular location
Cytoplasm
Source
KLH conjugated synthetic peptide derived from human NCF1
Gene ID number
653361
Swiss Prot
P14598
Tested applications
IF(IHC-P)
Recommended dilutions
IF(IHC-P)(1:50-200)
Crossreactivity
Human, Mouse, Rat
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Purification method
This antibody was purified via Protein A.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.