Long name NCF1/p47 phox(Ser359) Antibody, FITC Conjugated
Category Conjugated Primary Antibodies
Host Organism Rabbit (Oryctolagus cuniculus)
Target Antigen NCF1/p47 phox Ser359
Specificity This is a highly specific antibody against NCF1/p47 phox Ser359.
Modification site Ser359
Clone Polyclonal antibody
Concentration 1ug per 1ul
Source KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359
Gene ID number 653361
Tested applications IF(IHC-P)
Recommended dilutions IF(IHC-P)(1:50-200)
Crossreactivity Human, Rat
Crossreactive species details Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Antigen background The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
Purification method This antibody was purified via Protein A.
Storage conditions Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Properties If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.